Pgemt Vector, supplied by Promega, used in various techniques. What's in the box? The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. Instructions for Use of Product(s) E1910, E1960. Bioz Stars score: 97/100, based on 572 PubMed citations. Falls Sie über einen defekten Link hierher gekommen sind, schreiben Sie bitte eine E-Mail an den Webmaster (webmaster@promega.cc).Falls es … Designed to work seamlessly with Promega assays, GloMax® microplate readers and luminometers come preloaded with protocols that allow you to run assays at the touch of a button. Use the DNA molecular weight markers for both conventional and pulsed field gel electrophoresis applications. • Blue/white screening for recombinants. Incubate at room temperature for 1 hour or overnight at 4°C 3. Acids Res. • In vitro transcription from dual-opposed promoters. Contact Technical Services. Schweden. These single-nucleotide overhangs anneal to the 3´‑thymidine overhangs of T-cloning vectors such as the Promega pGEM®-T (Cat.# A3600) and pGEM®-T Easy Vectors (Cat.# A1360) and the Invitrogen TOPO® TA Cloning Kit with pCR®2.1-TOPO® Vector (Invitrogen Cat.# K4500-01). Company Promega; Price Supplier Page View Company Product Page; Catalog Number A3610; Quantity 20 reactions; Vector Name pGEM®-T Vector; Applications • Cloning PCR products. E151A: 1 × 1 vial: Passive Lysis Buffer, 5X. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 97/100, based on 2 PubMed citations. プロメガ（Promega Corporation）はライフサイエンスの分野に生化学および分子生物学に関連する革新的で価値の高い製品を世界の研究者にお届けするリーディングカンパニーです。 Dual-Luciferase Reporter Assay and Dual-Luciferase Reporter 1000 Assay System Quick Protocol FB024 PDF (73 KB) – English. Vector Pgemt Easy, supplied by Promega, used in various techniques. and Graham, A. The luminescence signal from these reporter vectors is used as an indicator of the activity of any gene/DNA element fused to the reporter. Bioz Stars score: 99/100, based on 161 PubMed citations. GoTaq® Green Master Mix is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. Item Part # Size Available Separately; Luciferase Assay Substrate. Add to the reaction mixture: a. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 161 PubMed citations. 16, 9677–86. プロトコル クイックプロトコル (pGEM-T Vectors) 製品マニュアル. Get cells out of freezer, thaw on ice for 5 minutes 2. Purified DNA is suitable for amplifications, restriction enzyme digestion and membrane hybridizations (e.g., Southern and dot/slot blots). The Global Reporter Gene Assay Kits market exhibits comprehensive information that is a valuable source of insightful data for business strategists during the decade 2014-2027. Promega AG wurde in der Schweiz 1991 unter dem Namen Catalys AG gegründet. Instructions for Use of Product(s) M7121: GoTaq Green Master Mix, M7122, M7123 Literature # 9PIM712. A protocol for isolation of genomic DNA from white blood cells, tissue culture cells, animal and plant tissue, yeast, Gram-positive and Gram-negative bacteria. Cloning - Promega pGEM-T Easy kit Ligation 1. Pgemt Promega Polylinker, supplied by Promega, used in various techniques. e-mail: office@promega.cc homepage: www.promega.cc Filmenbuchgericht: Feldkirch Filmenbuch: FN 336296d UST-IdNr. Als Tochtergesellschaft von Promega Corp. bietet die Schweizer Niederlassung in Dübendorf zusätzlich und komplementär zu Produkten von Promega Corp. auch Labor- und Analysegeräte sowie Verbrauchsmaterialien der Firmen Serva GmbH an. Specifications. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. (1988) Nucl. Technical Manual pGEM®-T and pGEM®-T Easy Vector Systems カタログカタログカタログ番号カタログ番号番号番号 A1360, A1380, A3600, A3610 www.promega.co.jp 注意注意注意：注意：：： この日本語マニュアルは製品に添付される英文マニュアルを翻訳したもの 3 µl Fresh PCR product b. Read More About Sensitivity. (2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. • Production of ssDNA. • Construction of unidirectional nested deletions with the Erase-a-Base® System. ATU65297145 ARA -Nummer: 17418 PROMEGA Handelsgesellschaft m.b.H Treietstraße 1 A-6833 Klaus Seite nicht gefunden. The pGEM-T and pGEM-T Easy Systems now allow you to perform ligation reactions in as little as one hour. Literature # TM040. Video Protocols. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. Protein expression vectors are basic tools for production of proteins. Page 4 Revised 5/07 GGAGA GCTCC CAACG CGTTG GATGC ATAGC TTGAG TATTC TATAG … Theoretically, smaller inserts will be cloned more efficiently than longer inserts. Complete Protocol. Eukaryotische Expressionssysteme Analyse der Top-Unternehmen 2021 – Thermo Fisher, Agilent, New England Biolabs, Sigma Aldrich, Promega apexresearch März 9, 2021 Nischenmarktforschungsbericht 2021 über Eukaryotische Expressionssysteme Markt mit Branchenwachstumsanalyse, Wettbewerb, Trends und Prognose bis 2026. Theoretically, smaller inserts will be cloned more efficiently than longer inserts. (1994) In: PCR, BIOS Scientific Publishers, Ltd., Oxford, UK, 13. Choose from a range of DNA, RNA and protein molecular weight markers. Promega AG beschäftigt 16 Mitarbeiter. Pgemt Easy Vector, supplied by Promega, used in various techniques. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid. Die Promega … • Construction of unidirectional nested deletions with the Erase-a-Base® System. Company Promega; Price Supplier Page View Company Product Page; Catalog Number A3600; Quantity 20 reactions; Vector Name pGEM®-T Vector; Applications • Cloning PCR products. DA: 59 PA: 43 MOZ Rank: 15. Store on ice until needed Transformation 1. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour ligation reactions. • Production of ssDNA. These single-nucleotide overhangs anneal to the 3´‑thymidine overhangs of T-cloning vectors such as the Promega pGEM®-T (Cat.# A3600) and pGEM®-T Easy Vectors (Cat.# A1360) and the Invitrogen TOPO® TA Cloning Kit with pCR®2.1-TOPO® Vector (Invitrogen Cat.# K4500-01). 5 µl 2x Rapid Ligation Buffer c. 1 µl pGEM-T vector d. 1 µl T4 DNA Ligase 2. Promega’s pGEM-T vector systems are very dependable and versatile tools to routinely clone fragments of DNA into reliable plasmids. The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. pGEMt_vector - Free download as PDF File (.pdf), Text File (.txt) or read online for free. The pGEM-T and pGEM-T Easy plasmid vectors are essentially the same but with one important difference. Quick Protocols. 1/21 Apoptosis Assays ProductSize Cat.# Caspase-Glo® 3/7 Assay System 2.5ml G8090 Caspase-Glo® 3/7 3D Assay System 10ml G8981 RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay 100 assays JA1011 Additional … Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. Bioz Stars score: 97/100, based on 2 PubMed citations. • In vitro transcription from dual-opposed promoters. Pgemt, supplied by Promega, used in various techniques. Protocols. Clark, J.M. Many Promega Vectors are reporter vectors containing various combinations of luciferase genes (firefly, Renilla, NanoLuc luciferase) with other genetic elements. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 16 PubMed citations. The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, and contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Robles, J. and Doers, M. (1994) Promega Notes 45, 19–20. 製品マニュアル（日本語） DH5α使用説明書. On the basis of historical data, Reporter Gene Assay Kits market report provides key segments and their sub-segments, revenue and demand & supply data. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 97/100, based on 572 PubMed citations. “Reporter Gene Assay Kits Market Scenario 2020-2027:. The product portfolio includes RNA purification solutions for all sample throughput needs, from manual purification to high-throughput automated systems. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 177 PubMed citations. ベクターマップ＆シークエンス Related Posts. The pGEM-T Easy vector has EcoRI restriction sites surrounding the proposed insert site, whereas the pGEM-T vector does not. Luminometer and Microplate Reader Selection Guide. Promega Technical Services Scientists are here to assist you in troubleshooting your experiments at any time. Bioz Stars score: 99/100, based on 16 PubMed citations. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual PDF (548 KB) – English. • Blue/white screening for recombinants. Bioz Stars score: 99/100, based on 177 PubMed citations. You are viewing: E1910 Change Configuration. Newton, C.R. Promega RNA purification products extract high-quality total RNA, mRNA or miRNA from many sample types, including blood, cells, tissues, FFPE tissue and total RNA preparations. Die von Ihnen gewünschte Seite kann nicht gefunden werden, oder ist momentan nicht erreichbar. Cloning with pGEM®-T Vectors: Ligation; January 8, 2020; A Quick Method for A Tailing PCR Products; July 8, 2019; T-Vector Cloning: Questions, Answers and Tips ; November 12, 2019; The following two tabs change content below. www.promega.com Part# TM042 Printed in USA.

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